Genetic modification of baculovirus expression vectors
نویسندگان
چکیده
منابع مشابه
Baculovirus expression vectors with single strand capability.
Recombinant baculoviruses offer a useful way of expressing eucaryotic genes, often in high yield (1). Current procedures for the generation of a recombinant virus make use of a transfer vector that, via recombination in vivo, place the gene of interest under control of the powerful polyhedrin promotor. At present the expression of mutant forms of the cloned gene (to study structure and function...
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Gene therapy has the potential to treat devastating inherited diseases for which there is little hope of finding a conventional cure and is a method for the prevention, correction, or modulation of genetic and acquired diseases that uses genes to therapeutic proteins. Animal viral systems are by far the most effective means of gene delivery and achieve high efficiencies for both delivery and ex...
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ATP-binding cassette (ABC) drug transporters ABCB1 [P-glycoprotein (Pgp)] and ABCG2 are expressed in many tissues including those of the intestines, the liver, the kidney and the brain and are known to influence the pharmacokinetics and toxicity of therapeutic drugs. In vitro studies involving their functional characteristics provide important information that allows improvements in drug delive...
متن کاملLinearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors.
Engineered derivatives of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV) possessing a unique restriction site provide a source of viral DNA that can be linearized by digestion with a specific endonuclease. Circular or linearized DNA from two such viruses were compared in terms of their infectivity and recombinogenic activities. The linear forms were 15- to 150-...
متن کاملNovel baculovirus expression vectors that provide sialylation of recombinant glycoproteins in lepidopteran insect cells.
This report describes novel baculovirus vectors designed to express mammalian beta1,4-galactosyltransferase and alpha2,6-sialyltransferase genes at early times after infection. Sf9 cells infected with these viral vectors, unlike cells infected with a wild-type baculovirus, produced a sialylated viral glycoprotein during the late phase of infection. Thus, the two mammalian glycosyltransferases e...
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ژورنال
عنوان ژورنال: Virologica Sinica
سال: 2012
ISSN: 1674-0769,1995-820X
DOI: 10.1007/s12250-012-3236-y